Aberration free refocusing for high numerical aperture microscopy

Edward Botcherby; Rimas Juškaitis; Martin Booth; Tony Wilson

doi:10.1117/12.771419

12 February 2008 Aberration free refocusing for high numerical aperture microscopy

Edward Botcherby, Rimas Juškaitis, Martin Booth, Tony Wilson

Proceedings Volume 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV; 686110 (2008) https://doi.org/10.1117/12.771419
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

In this paper, we present a novel technique that permits sectioning microscopes to refocus and acquire images from a large range of specimen depths without introducing movements near the specimen. In contrast to other such remote focusing methods, this technique avoids systematic aberrations that degrade image quality when imaging planes away from the optimal focal plane. Furthermore, the specific geometry that is employed in this work enables refocusing to be carried out at high speed and hence permits, for the first time, a number of dynamical biological processes to be observed. Although this technique can be applied to any optical imaging system, it is particularly suited to the case of high numerical aperture microscope systems. To demonstrate this we present results from two prototype systems built in our laboratory based on a slit scanning confocal fluorescence microscope and a two photon fluorescence microscope.

Citation Download Citation

Edward Botcherby, Rimas Juškaitis, Martin Booth, and Tony Wilson "Aberration free refocusing for high numerical aperture microscopy", Proc. SPIE 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV, 686110 (12 February 2008); https://doi.org/10.1117/12.771419

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