A calibrated tissue phantom for small animal fluorescence imaging

Silas Leavesley; J. Paul Robinson

doi:10.1117/12.763125

21 February 2008 A calibrated tissue phantom for small animal fluorescence imaging

Silas Leavesley, J. Paul Robinson

Proceedings Volume 6870, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue; 68700C (2008) https://doi.org/10.1117/12.763125
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Tissue phantoms have been in use for decades in the quality control and calibration of x-ray, computed tomography, magnetic resonance imaging, and positron emission tomography. Such phantoms are necessary in clinical imaging, insuring the accuracy and calibration of acquired images and allowing for artifact correction. In addition, phantoms allow image comparison and/or registration between multiple instruments at different clinics and temporal comparison of the same animal. Recently, optical tissue phantoms have received much attention as it has become apparent that optical imagers pose many of the same requirements for quality control and calibration as clinical imagers. Small animal fluorescence imaging is a rapidly growing field that could benefit greatly from the implementation of a standardized reference phantom. We present our results in developing optical tissue phantoms for quality control and calibration of small animal fluorescence imagers. To accurately simulate in vivo imaging conditions, the phantom provides absorption, scatter, and fluorescence information, in known amounts and varying with spatial distribution. This allows us to monitor day-to-day variability and system response as a function of different optical path components in hyperspectral imaging technologies for small animal fluorescence imaging. The phantom has also enabled us to assess the actual, or practical, sensitivity of a small animal fluorescence imager in terms of imaging a dye concentration through an equivalent tissue depth. By combining an understanding of system response with theoretical optical raytrace modeling, this calibration tool can be applied to the majority of small animal fluorescence imagers currently in production.

Citation Download Citation

Silas Leavesley and J. Paul Robinson "A calibrated tissue phantom for small animal fluorescence imaging", Proc. SPIE 6870, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue, 68700C (21 February 2008); https://doi.org/10.1117/12.763125

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KEYWORDS

Luminescence

Tissue optics

Imaging systems

Calibration

Tissues

Preclinical imaging

Animal model studies

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