Nano-aquarium for dynamic observation of aquatic microorganisms fabricated by femtosecond laser direct writing of photostructurable glass

Y. Hanada; K. Sugioka; H. Kawano; I. Ishikawa; A. Miyawaki M.D.; K. Midorikawa

doi:10.1117/12.762391

23 February 2008 Nano-aquarium for dynamic observation of aquatic microorganisms fabricated by femtosecond laser direct writing of photostructurable glass

Y. Hanada, K. Sugioka, H. Kawano, I. Ishikawa, A. Miyawaki M.D., K. Midorikawa

Author Affiliations +

Proceedings Volume 6880, Laser-based Micro- and Nanopackaging and Assembly II; 68800E (2008) https://doi.org/10.1117/12.762391
Event: Lasers and Applications in Science and Engineering, 2008, San Jose, California, United States

Abstract

We demonstrate the fabrication of three-dimensional (3-D) hollow microstructures embedded in photostructurable glass by a femtosecond (fs) laser direct writing. Fs laser direct writing followed by annealing and successive wet etching in dilute hydrofluoric (HF) acid solution resulted in the rapid manufacturing of microchips with 3-D hollow microstructures for the dynamic observation of living microorganisms in fresh water. The embedded microchannel structure enables us to analyze the continuous motion of Euglena gracilis. A microchamber with a movable microneedle demonstrates its ability for the elucidation of the information transmission process in Pleurosira laevis. Such microchips, referred to as nano-aquariums realize the efficient and highly functional observation of microorganisms.

Citation Download Citation

Y. Hanada, K. Sugioka, H. Kawano, I. Ishikawa, A. Miyawaki M.D., and K. Midorikawa "Nano-aquarium for dynamic observation of aquatic microorganisms fabricated by femtosecond laser direct writing of photostructurable glass", Proc. SPIE 6880, Laser-based Micro- and Nanopackaging and Assembly II, 68800E (23 February 2008); https://doi.org/10.1117/12.762391

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