Methods to directly measure the trapping potential in optical tweezers

Arvind Balijepalli; Thomas W. Lebrun; Jason J. Gorman; Satyandra K. Gupta

doi:10.1117/12.796513

29 August 2008 Methods to directly measure the trapping potential in optical tweezers

Arvind Balijepalli, Thomas W. Lebrun, Jason J. Gorman, Satyandra K. Gupta

Proceedings Volume 7038, Optical Trapping and Optical Micromanipulation V; 70380V (2008) https://doi.org/10.1117/12.796513
Event: NanoScience + Engineering, 2008, San Diego, California, United States

Abstract

Techniques to measure the trapping force in an optical tweezers without any prior assumptions about the trap shape have been developed. The response of a trapped micro or nanoparticle to a step input is measured and then used to calculate the trapping force experienced by the particle as a function of it's position in the trap. This method will provide new insight into the trapping behavior of nanoparticles, which are more weakly bound than microparticles and thereby explore larger regions of the trapping potential due to Brownian motion. Langevin dynamics simulations are presented to model the system and are used to demonstrate this technique. Preliminary experimental results are then presented to validate the simulations. Finally, the measured trapping forces, from simulations and laboratory experiments, are integrated to recover the trapping potential.

Citation Download Citation

Arvind Balijepalli, Thomas W. Lebrun, Jason J. Gorman, and Satyandra K. Gupta "Methods to directly measure the trapping potential in optical tweezers", Proc. SPIE 7038, Optical Trapping and Optical Micromanipulation V, 70380V (29 August 2008); https://doi.org/10.1117/12.796513

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