First derivative of NIR light diffuse reflectance spectra as an approach to analyze muscle tissue chromophores and light pathlength

Eugene Gussakovsky

doi:10.1117/12.807716

24 February 2009 First derivative of NIR light diffuse reflectance spectra as an approach to analyze muscle tissue chromophores and light pathlength

Eugene Gussakovsky

Author Affiliations +

Proceedings Volume 7187, Biomedical Applications of Light Scattering III; 71871D (2009) https://doi.org/10.1117/12.807716
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Diffuse reflectance was applied to the biomedical studies (muscles, cardiac tissues etc.) in a form of either a direct pseudo-optical spectrum or its second derivative. The first derivative adopts advantages of both direct spectrum (high signal-to-noise ratio) and its second derivative (simplifying the consideration of light scattering contribution, S). In contrast to spectrophotometry of solutions, diffuse reflectance application to the analysis of turbid medium chromophores leads to non-trivial problems of contribution of light scattering, the choice of reference, and light pathlength. Under certain conditions, the first approximation of the Taylor series of S results in the known linear dependence of S on wavelength in the 650-1050 nm wavelength range. Then the light scattering contribution to the first derivative becomes a wavelength-independent offset. In contrast to the second derivative, the information on light scattering inside the tissue is not lost. Effect of reference on the measured spectra becomes negligible. Application of the first derivative allowed (i) determination of NIR light pathlength in muscle tissue, and (ii) quantification of hemoglobin + myoglobin absolute concentration (in mM) in cardiac tissue during open-heart surgery. The first derivative approach may in general be applied to any chromophores in turbid (biological) media.

Citation Download Citation

Eugene Gussakovsky "First derivative of NIR light diffuse reflectance spectra as an approach to analyze muscle tissue chromophores and light pathlength", Proc. SPIE 7187, Biomedical Applications of Light Scattering III, 71871D (24 February 2009); https://doi.org/10.1117/12.807716

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available