Thorben Cordes,1 Ingo H. Stein,1 Carsten Forthmann,1 Christian Steinhauer,1 Monika Walz,1 Wolfram Summerer,1 Britta Person,1 Jan Vogelsang,1 Philip Tinnefeld1
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In this paper we show that the emission of ordinary organic dyes can be controlled in order to increase photostability or
to induce long off-states for superresolution microscopy. We therefore extend a recently introduced concept that utilizes
triplet-state quenching via redox-reactions and recovery of the electronic ground-state by complementary redoxreactions:
it is shown that different reagents in an oxidizing and reducing system (ROXS) can positively influence the
fluorescence properties of organic dyes. In more detail, the effects of Trolox, a ferrocene-based compound, an oxidized
quinone derivative of Trolox and nitrobenzoic acid are investigated and compared to the prototypical compounds
ascorbic acid and N,N methylviologen. While the redox potential is the most important parameter for the realization of
the ROXS concept it is demonstrated that also kinetic aspects have to be taken into account to explain the properties of
the specific redox agents. Photostabilization and the induction of off-states are of paramount importance for fluorescence
microscopy in general and especially for superresolution microscopy based on "blinking" molecules.
Thorben Cordes,Ingo H. Stein,Carsten Forthmann,Christian Steinhauer,Monika Walz,Wolfram Summerer,Britta Person,Jan Vogelsang, andPhilip Tinnefeld
"Controlling the emission of organic dyes for high sensitivity and super-resolution microscopy", Proc. SPIE 7367, Advanced Microscopy Techniques, 73671D (1 July 2009); https://doi.org/10.1117/12.831495
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Thorben Cordes, Ingo H. Stein, Carsten Forthmann, Christian Steinhauer, Monika Walz, Wolfram Summerer, Britta Person, Jan Vogelsang, Philip Tinnefeld, "Controlling the emission of organic dyes for high sensitivity and super-resolution microscopy," Proc. SPIE 7367, Advanced Microscopy Techniques, 73671D (1 July 2009); https://doi.org/10.1117/12.831495