Sum frequency generation spectroscopy of amyloid fibrils and oligomers at air/water interface

Gediminas Niaura; Rima Budvytyte; Zenonas Kuprionis; Gintaras Valincius

doi:10.1117/12.871042

24 November 2010 Sum frequency generation spectroscopy of amyloid fibrils and oligomers at air/water interface

Gediminas Niaura, Rima Budvytyte, Zenonas Kuprionis, Gintaras Valincius

Proceedings Volume 7376, Laser Applications in Life Sciences; 73760Q (2010) https://doi.org/10.1117/12.871042
Event: Laser Applications in Life Sciences 2010, 2010, Oulu, Finland

Abstract

Structure of beta-amyloid oligomers and fibrils at air/water interface was studied by sum frequency generation (SFG) spectroscopy. It was found that both small (1-3 nm) and large (4-10 nm) oligomers as well as fibrils adsorb in ordered fashion at air/water interface. Intense resonances from methyl group stretching vibrations at 2875 and 2937 cm^-1 and "ice-like" structured water bands were characteristic for adsorbed fibrils. Similar spectral pattern was observed for large oligomers. However, small oligomers exhibited intense SFG resonance near 2912 cm^-1 due to methylene or methine C-H stretching vibration. No "ice-like" water band was observed in this case. The possibility of discrimination between the small (toxic) oligomers, large (non-toxic) oligomers and fibrils was demonstrated.

Citation Download Citation

Gediminas Niaura, Rima Budvytyte, Zenonas Kuprionis, and Gintaras Valincius "Sum frequency generation spectroscopy of amyloid fibrils and oligomers at air/water interface", Proc. SPIE 7376, Laser Applications in Life Sciences, 73760Q (24 November 2010); https://doi.org/10.1117/12.871042

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