Fluorescence Dynamics Of Calcium-Binding Proteins

Robert F. Steiner; Lynn Norris

doi:10.1117/12.966939

1 January 1987 Fluorescence Dynamics Of Calcium-Binding Proteins

Robert F. Steiner, Lynn Norris

Proceedings Volume 0743, Fluorescence Detection; (1987) https://doi.org/10.1117/12.966939
Event: OE LASE'87 and EO Imaging Symposium, 1987, Los Angeles, CA, United States

Abstract

The time decay of fluorescence intensity and anisotropy has been measured for an N-acetylaminoethyl-5-naphthylamine-l-sulfonate probe attached to Cys-98 of troponin C, a dansyl aziridine label linked to Met-25 of troponin C, and 2-p-toluidinyl naphthalene-6- sulfonate bound by calmodulin. In all cases the time decay of intensity at 25° was multi-exponential. The time decay of fluorescence anisotropy was also multi-exponential and could be fitted in terms of a long correlation time reflecting the rotational motion of all, or a major portion of the molecule, and a short correlation time arising from a more localized motion. For the Ca²⁺-liganded forms of both proteins the magnitude of the longer correlation time could be accounted for in terms of the structure of native calmodulin under these conditions. In the case of the label linked to Cys-98 of troponin C in the absence of Ca²⁺, the probe senses the rotation of a subelement of the molecule at 25°; at 40° the probe rotates almost independently of the overall structure.

Citation Download Citation

Robert F. Steiner and Lynn Norris "Fluorescence Dynamics Of Calcium-Binding Proteins", Proc. SPIE 0743, Fluorescence Detection, (1 January 1987); https://doi.org/10.1117/12.966939

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