Light scattering modeling of bacteria using spheroids and cylinders

Chunxia Feng; Lihua Huang; Jie Han; Guangchao Zhou; Aijun Zeng; Yongkai Zhao; Huijie Huang

doi:10.1117/12.837919

20 November 2009 Light scattering modeling of bacteria using spheroids and cylinders

Chunxia Feng, Lihua Huang, Jie Han, Guangchao Zhou, Aijun Zeng, Yongkai Zhao, Huijie Huang

Proceedings Volume 7511, 2009 International Conference on Optical Instruments and Technology: Optoelectronic Measurement Technology and Systems; 75110P (2009) https://doi.org/10.1117/12.837919
Event: International Conference on Optical Instrumentation and Technology, 2009, Shanghai, China

Abstract

Numerical simulations of light scattering by irregularly shaped bacteria are carried out using the T-matrix method. A previously developed T-matrix code for the study of light scattering by randomly oriented non-spherical particles is used for the current purpose and it is validated against Mie-theory using coccus. Simplified particle shapes of spheroids and cylinders for simulating scattering by irregularly shaped bacteria are studied. The results for the angular distributions of the scattering matrix elements of B.Subtilis at wavelength 0.6328μm are presented. Their dependence on shape and model are discussed. Analysis suggests that spheroids perform better than cylinders for B.Subtilis. Calculations of the scatter matrix elements to determine bacteria sizes as well as shapes may be an accurate method and may be used to determine what the bacteria are.

Citation Download Citation

Chunxia Feng, Lihua Huang, Jie Han, Guangchao Zhou, Aijun Zeng, Yongkai Zhao, and Huijie Huang "Light scattering modeling of bacteria using spheroids and cylinders", Proc. SPIE 7511, 2009 International Conference on Optical Instruments and Technology: Optoelectronic Measurement Technology and Systems, 75110P (20 November 2009); https://doi.org/10.1117/12.837919

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