Four-dimensional multi-site two-photon excitation

Vincent R. Daria; Christian Stricker; Richard Bowman; Hans-A. Bachor; Stephen Redman

doi:10.1117/12.840782

2 March 2010 Four-dimensional multi-site two-photon excitation

Vincent R. Daria, Christian Stricker, Richard Bowman, Hans-A. Bachor, Stephen Redman

Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 75483T (2010) https://doi.org/10.1117/12.840782
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We use the holographic method to project an arbitrary array of diffraction-limited focal spots suitable for multi-site twophoton excitation. The spot array can be projected arbitrarily within a three-dimensional (3D) volume, while the fourth dimension in time is attributed to high temporal resolution via high-speed non-iterative calculation of the hologram using a video graphics accelerator board. We show that the spots have sufficient energy and spatiotemporal photon density for localized two-photon excitation at individual spots in the array. The significance of this work points to 3D microscopy, non-linear micro-fabrication, volume holographic optical storage and biomedical instrumentation. In neuroscience, timecritical release of neurotransmitters at multiple sites within complex dendritic trees of neurons can lead to insights on the mechanisms of information processing in the brain.

Citation Download Citation

Vincent R. Daria, Christian Stricker, Richard Bowman, Hans-A. Bachor, and Stephen Redman "Four-dimensional multi-site two-photon excitation", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75483T (2 March 2010); https://doi.org/10.1117/12.840782

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