Polarized Raman spectroscopy of bone tissue: watch the scattering

Mekhala Raghavan; Nadder D. Sahar; Robert H. Wilson; Mary-Ann Mycek; Nancy Pleshko; David H. Kohn; Michael D. Morris

doi:10.1117/12.841977

2 March 2010 Polarized Raman spectroscopy of bone tissue: watch the scattering

Mekhala Raghavan, Nadder D. Sahar, Robert H. Wilson, Mary-Ann Mycek, Nancy Pleshko, David H. Kohn, Michael D. Morris

Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 754848 (2010) https://doi.org/10.1117/12.841977
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Polarized Raman spectroscopy is widely used in the study of molecular composition and orientation in synthetic and natural polymer systems. Here, we describe the use of Raman spectroscopy to extract quantitative orientation information from bone tissue. Bone tissue poses special challenges to the use of polarized Raman spectroscopy for measurement of orientation distribution functions because the tissue is turbid and birefringent. Multiple scattering in turbid media depolarizes light and is potentially a source of error. Using a Raman microprobe, we show that repeating the measurements with a series of objectives of differing numerical apertures can be used to assess the contributions of sample turbidity and depth of field to the calculated orientation distribution functions. With this test, an optic can be chosen to minimize the systematic errors introduced by multiple scattering events. With adequate knowledge of the optical properties of these bone tissues, we can determine if elastic light scattering affects the polarized Raman measurements.

Citation Download Citation

Mekhala Raghavan, Nadder D. Sahar, Robert H. Wilson, Mary-Ann Mycek, Nancy Pleshko, David H. Kohn, and Michael D. Morris "Polarized Raman spectroscopy of bone tissue: watch the scattering", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 754848 (2 March 2010); https://doi.org/10.1117/12.841977

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available