Ex vivo hemoglobin status study using photoacoustic computed tomography small animal scanner

Bo Liu; Robert Kruger; Daniel Reinecke; Keith M. Stantz

doi:10.1117/12.842836

23 February 2010 Ex vivo hemoglobin status study using photoacoustic computed tomography small animal scanner

Bo Liu, Robert Kruger, Daniel Reinecke, Keith M. Stantz

Proceedings Volume 7564, Photons Plus Ultrasound: Imaging and Sensing 2010; 75643A (2010) https://doi.org/10.1117/12.842836
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Purpose: The purpose of this study is to calibrate the PCT scanner to quantify the hemoglobin status utilizing a blood flow phantom. Materials and Methods: A blood circulation system was designed and constructed to control the oxygen saturation and hemoglobin concentration of blood. As a part of the circulation system, a 1.1mm FEP tube was placed in the center of imaging tank of PCT scanner as the imaging object. Photoacoustic spectra (690-950 nm) was acquired for different hemoglobin concentrations (CtHb) and oxygen saturation levels (SaO2), where the formers was formed by diluting blood samples with PBS and the latter by mixing blood with gases at different oxygen content. Monte Carlo simulations were performed to calculate the photon energy depositions in the phantom tube, which took into account photon losses in water and blood. A Kappa value which represents the energy transfer efficiency of hemoglobin molecule was calculated based on the PCT measurement and simulation result. The final SaO2 value of each blood sample was calculated based on the PCT spectrum and Kappa value. These oxygen saturation results were compared with co-oximeter measurements to obtain systematic errors. Results and Conclusion: The statistic error of calculating Kappa value from hemoglobin concentration experiment was less than 5%. The systematic error between PCT spectra analysis and co-oximeter analysis for hemoglobin oxygen saturation was -4.5%. These calibration techniques used to calculate Kappa and hemoglobin absorption spectra would be used in hypoxia measurements in tumors as well as for endogenous biomarkers studies.

Citation Download Citation

Bo Liu, Robert Kruger, Daniel Reinecke, and Keith M. Stantz "Ex vivo hemoglobin status study using photoacoustic computed tomography small animal scanner", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75643A (23 February 2010); https://doi.org/10.1117/12.842836

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available