Adaptive multiphoton and harmonic generation microscopy for developmental biology

Anisha Thayil; Alexander Jesacher; Tomoko Watanabe; Tony Wilson; Shankar Srinivas; Martin J. Booth

doi:10.1117/12.843312

26 February 2010 Adaptive multiphoton and harmonic generation microscopy for developmental biology

Anisha Thayil, Alexander Jesacher, Tomoko Watanabe, Tony Wilson, Shankar Srinivas, Martin J. Booth

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75691I (2010) https://doi.org/10.1117/12.843312
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Specimen-induced aberrations often affect microscopes, particularly when high numerical aperture lenses are used to image deep into biological specimens. These aberrations cause a reduction in resolution and focal intensity. This is particularly problematic in multiphoton microscopy, where the non-linear nature of the signal generation process means that the signal level is strongly affected by changes in the focal spot intensity. We have applied the techniques of adaptive optics to correct aberrations in two-photon fluorescence and harmonic generation microscopes, restoring image quality. In particular we have used these microscopes for studies in developmental biology and for the imaging of mammalian embryos.

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Anisha Thayil, Alexander Jesacher, Tomoko Watanabe, Tony Wilson, Shankar Srinivas, and Martin J. Booth "Adaptive multiphoton and harmonic generation microscopy for developmental biology", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75691I (26 February 2010); https://doi.org/10.1117/12.843312

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KEYWORDS

Microscopes

Harmonic generation

Microscopy

Second-harmonic generation

Adaptive optics

Aberration correction

Monochromatic aberrations

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