Three-dimensional single particle tracking using off-axis digital holographic microscopy

Yoonsung Bae; Seungrag Lee; Wenzhong Yang; Dug Y. Kim

doi:10.1117/12.842882

12 February 2010 Three-dimensional single particle tracking using off-axis digital holographic microscopy

Yoonsung Bae, Seungrag Lee, Wenzhong Yang, Dug Y. Kim

Proceedings Volume 7574, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VII; 757408 (2010) https://doi.org/10.1117/12.842882
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Three dimensional particle tracking is useful technology to characterize live cell or surrounding environment by tracing small particles such as fluorescence beads or polystyrene beads which adhered to objective samples. In microscopy imaging system, the longitudinal(z axis) tracking of the particle is essential for implementation of three-dimensional particle tracking, however it's been still a challenging topic to find the exact position of the particle in z axis with high precision. In this study, we present that a novel technique to find the longitudinal position of the particle, as well as the transverse position(x,y axis) by applying the numerical reconstruction and focusing with digital holographic microscope. Transmission type off-axis digital holographic microscope is implemented for this experiment, based on Mach-Zehnder interferometer and 632.8nm HeNe laser is used as a coherent light source of the microscope and high-speed CMOS camera is utilized for acquiring the hologram. Digital holographic microscope makes it possible to record and reconstruct the phase and amplitude image of the sample. In order to find the position of the particle in z axis, we apply the numerical focusing algorithm, which enables the translation of the imaging focus without actual longitudinal movement of the sample. To demonstrate the presented method, Brownian movement of 3μm polystyrene sphere suspended in water is investigated in this experiment.

Citation Download Citation

Yoonsung Bae, Seungrag Lee, Wenzhong Yang, and Dug Y. Kim "Three-dimensional single particle tracking using off-axis digital holographic microscopy", Proc. SPIE 7574, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VII, 757408 (12 February 2010); https://doi.org/10.1117/12.842882

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available