Dynamical deformation compensation of phase in digital holographic microscopy

Tomáš Zikmund; Lukáš Kvasnica; Hana Uhlířová; Luděk Lovicar; Radim Chmelík

doi:10.1117/12.882171

14 December 2010 Dynamical deformation compensation of phase in digital holographic microscopy

Tomáš Zikmund, Lukáš Kvasnica, Hana Uhlířová, Luděk Lovicar, Radim Chmelík

Proceedings Volume 7746, 17th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics; 77460K (2010) https://doi.org/10.1117/12.882171
Event: 17th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 2010, Liptovsky Jan, Slovakia

Abstract

Digital holographic microscopy (DHM) is a powerful tool for observation of biological samples. The main advantage of DHM imaging is the possibility to reconstruct the intensity image and the phase image of an observed sample in real-time. The background of phase images is deformed and this deformation is changing during a long-term experiment. We describe here a novel procedure of deformation compensation for sequence of phase images. This novel procedure is based on deformation compensation procedure for a single phase image which uses the least square method. The novel procedure is applicable to compensate deformation during observation in real-time. The proposed procedure is applied to phase images of cells obtained by a transmitted-light digital holographic microscope.

Citation Download Citation

Tomáš Zikmund, Lukáš Kvasnica, Hana Uhlířová, Luděk Lovicar, and Radim Chmelík "Dynamical deformation compensation of phase in digital holographic microscopy", Proc. SPIE 7746, 17th Slovak-Czech-Polish Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 77460K (14 December 2010); https://doi.org/10.1117/12.882171

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