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An enzyme based biosensor was fabricated by employing a simple, inexpensive and rapid xurography fabrication
process. The electrodes and channel were made from the conducting polymer poly(3,4-ethyelenedioxythiphene)
poly(styrene sulfonate) (PEDOT:PSS). PEDOT:PSS was selectively deposited using a polyimide tape mask. The tape
mask was peeled off from the substrate after annealing the polymer in vacuum. Polymer wells of defined dimensions
were made and were attached to the device to accommodate the solutions. This sensor utilizes the change in current as a
parameter to measure different analyte concentrations. Initial experiments were done by using the sensor for glucose
detection. The sensor is able to detect the glucose concentrations approximately from 1 μM to 10 mM range covering
glucose in human saliva (8-210 μM). The glucose oxidase activity was independently measured using colorimetric
method and the results indicate that the sensor retains the enzyme activity and can be used as a biosensor to detect
various analytes. The analyte of interest can be measured by preloading the corresponding enzyme into the wells.
Senaka K. Kanakamedala,Haidar T. Alshakhouri,Mangilal Agarwal,Ji Fang, andMark A. DeCoster
"A simple enzyme based biosensor on flexible plastic substrate", Proc. SPIE 7759, Biosensing III, 77590F (24 August 2010); https://doi.org/10.1117/12.860587
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Senaka K. Kanakamedala, Haidar T. Alshakhouri, Mangilal Agarwal, Ji Fang, Mark A. DeCoster, "A simple enzyme based biosensor on flexible plastic substrate," Proc. SPIE 7759, Biosensing III, 77590F (24 August 2010); https://doi.org/10.1117/12.860587