Effect of clearing agents on scattering coefficient and anisotropy of scattering of dermis studied by reflectance confocal microscopy

S. L. Jacques; R. Samatham; K. G. Phillips

doi:10.1117/12.874060

11 February 2011 Effect of clearing agents on scattering coefficient and anisotropy of scattering of dermis studied by reflectance confocal microscopy

S. L. Jacques, R. Samatham, K. G. Phillips

Author Affiliations +

Proceedings Volume 7907, Biomedical Applications of Light Scattering V; 790706 (2011) https://doi.org/10.1117/12.874060
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Optical clearing of mouse dermis by glycerol was tested by reflectance-mode confocal microscopy (rCSLM) using 488- nm light. The reflectance signal R(z) was acquired as a function of the depth of the focus (z) within the upper 100 μm of freshly excised mouse dermis. The results specify the scattering coefficient (μs [cm_-1]) and the anisotropy of scattering (g [dimensionless]). The absorption is too low to exert an effect. The results, published in Samatham et al., Journal of Innovative Optical Health Sciences 2010, 3(3):183-188, described how the clearing effect of glycerol was to increase g toward nearly 1.0, while having only a modest effect on μ_s. In other words, glycerol caused light scattering to become very forward-directed, but did not strongly alter the number of scattering events per unit length of photon path. This paper discusses the possible mechanism of action that is responsible for this clearing effect.

Citation Download Citation

S. L. Jacques, R. Samatham, and K. G. Phillips "Effect of clearing agents on scattering coefficient and anisotropy of scattering of dermis studied by reflectance confocal microscopy", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 790706 (11 February 2011); https://doi.org/10.1117/12.874060

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