A dark-field scanning spectroscopy platform for localized scatter and fluorescence imaging of tissue

Venkataramanan Krishnaswamy; Ashley M. Laughney; Keith D. Paulsen; Brian W. Pogue

doi:10.1117/12.875462

11 February 2011 A dark-field scanning spectroscopy platform for localized scatter and fluorescence imaging of tissue

Venkataramanan Krishnaswamy, Ashley M. Laughney, Keith D. Paulsen, Brian W. Pogue

Proceedings Volume 7907, Biomedical Applications of Light Scattering V; 79070J (2011) https://doi.org/10.1117/12.875462
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Tissue ultra-structure and molecular composition provide native contrast mechanisms for discriminating across pathologically distinct tissue-types. Multi-modality optical probe designs combined with spatially confined sampling techniques have been shown to be sensitive to this type of contrast but their extension to imaging has only been realized recently. A modular scanning spectroscopy platform has been developed to allow imaging localized morphology and molecular contrast measures in breast cancer surgical specimens. A custom designed dark-field telecentric scanning spectroscopy system forms the core of this imaging platform. The system allows imaging localized elastic scatter and fluorescence measures over fields of up to 15 mm x 15 mm at 100 microns resolution in tissue. Results from intralipid and blood phantom measurements demonstrate the ability of the system to quantify localized scatter parameters despite significant changes in local absorption. A co-registered fluorescence spectroscopy mode is also demonstrated in a protophorphyrin-IX phantom.

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Venkataramanan Krishnaswamy, Ashley M. Laughney, Keith D. Paulsen, and Brian W. Pogue "A dark-field scanning spectroscopy platform for localized scatter and fluorescence imaging of tissue", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070J (11 February 2011); https://doi.org/10.1117/12.875462

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KEYWORDS

Spectroscopy

Luminescence

Fluorescence spectroscopy

Tissue optics

Imaging spectroscopy

Imaging systems

Tissues

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