In vivo laser assisted end-to-end anastomosis with ICG-infused chitosan patches

Francesca Rossi; Paolo Matteini; Giuseppe Esposito; Alba Scerrati; Alessio Albanese; Alfredo Puca; Giulio Maira; Giacomo Rossi; Roberto Pini

doi:10.1117/12.889800

2 June 2011 In vivo laser assisted end-to-end anastomosis with ICG-infused chitosan patches

Francesca Rossi, Paolo Matteini, Giuseppe Esposito, Alba Scerrati, Alessio Albanese, Alfredo Puca, Giulio Maira, Giacomo Rossi, Roberto Pini

Author Affiliations +

Proceedings Volume 8092, Medical Laser Applications and Laser-Tissue Interactions V; 809212 (2011) https://doi.org/10.1117/12.889800
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

Laser assisted vascular repair is a new optimized technique based on the use of ICG-infused chitosan patch to close a vessel wound, with or even without few supporting single stitches. We present an in vivo experimental study on an innovative end-to-end laser assisted vascular anastomotic (LAVA) technique, performed with the application of ICGinfused chitosan patches. The photostability and the mechanical properties of ICG-infused chitosan films were preliminary measured. The in vivo study was performed in 10 New Zealand rabbits. After anesthesia, a 3-cm segment of the right common carotid artery was exposed, thus clamped proximally and distally. The artery was then interrupted by means of a full thickness cut. Three single microsutures were used to approximate the two vessel edges. The ICG-infused chitosan patch was rolled all over the anastomotic site and welded by the use of a diode laser emitting at 810 nm and equipped with a 300 μm diameter optical fiber. Welding was obtained by delivering single laser spots to induce local patch/tissue adhesion. The result was an immediate closure of the anastomosis, with no bleeding at clamps release. Thus animals underwent different follow-up periods, in order to evaluate the welded vessels over time. At follow-up examinations, all the anastomoses were patent and no bleeding signs were documented. Samples of welded vessels underwent histological examinations. Results showed that this technique offer several advantages over conventional suturing methods: simplification of the surgical procedure, shortening of the operative time, better re-endothelization and optimal vascular healing process.

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Francesca Rossi, Paolo Matteini, Giuseppe Esposito, Alba Scerrati, Alessio Albanese, Alfredo Puca, Giulio Maira, Giacomo Rossi, and Roberto Pini "In vivo laser assisted end-to-end anastomosis with ICG-infused chitosan patches", Proc. SPIE 8092, Medical Laser Applications and Laser-Tissue Interactions V, 809212 (2 June 2011); https://doi.org/10.1117/12.889800

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KEYWORDS

Laser welding

In vivo imaging

Semiconductor lasers

Arteries

Laser tissue interaction

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Simulation of CCA and DLA aggregates

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