PH-sensitive fluorescence detection by diffuse fluorescence tomography

Jiao Li; Feng Gao; Linjing Duan; Xin Wang; Limin Zhang; Huijuan Zhao

doi:10.1117/12.905633

29 February 2012 PH-sensitive fluorescence detection by diffuse fluorescence tomography

Jiao Li, Feng Gao, Linjing Duan, Xin Wang, Limin Zhang, Huijuan Zhao

Proceedings Volume 8216, Multimodal Biomedical Imaging VII; 821606 (2012) https://doi.org/10.1117/12.905633
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

The importance of cellular pH has been shown clearly in the study of cell activity, pathological feature, drug metabolism, etc. Monitoring pH changes of living cells and imaging the regions with abnormal pH values in vivo could provide the physiologic and pathologic information for the research of the cell biology, pharmacokinetics, diagnostics and therapeutics of certain diseases such as cancer. Thus, pH-sensitive fluorescence imaging of bulk tissues has been attracting great attention in the regime of near-infrared diffuse fluorescence tomography (DFT), an efficient small-animal imaging tool. In this paper, the feasibility of quantifying pH-sensitive fluorescence targets in turbid medium is investigated using both time-domain and steady-state DFT methods. By use of the specifically designed time-domain and continuous-wave systems and the previously proposed image reconstruction scheme, we validate the method through 2-dimensional imaging experiments on a small-animal-sized phantom with multiply targets of distinct pH values. The results show that the approach can localize the targets with reasonable accuracy and achieve quantitative reconstruction of the pH-sensitive fluorescent yield.

Citation Download Citation

Jiao Li, Feng Gao, Linjing Duan, Xin Wang, Limin Zhang, and Huijuan Zhao "PH-sensitive fluorescence detection by diffuse fluorescence tomography", Proc. SPIE 8216, Multimodal Biomedical Imaging VII, 821606 (29 February 2012); https://doi.org/10.1117/12.905633

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KEYWORDS

Luminescence

Photon counting

Fluorescence tomography

Atrial fibrillation

Collimation

Imaging systems

Signal detection

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