Hydrogen peroxide induces apoptosis via a mitochondrial pathway in chondrocytes

Cai-ping Zhuang; Qian Liang; Xiao-ping Wang; Tong-sheng Chen

doi:10.1117/12.905786

13 February 2012 Hydrogen peroxide induces apoptosis via a mitochondrial pathway in chondrocytes

Cai-ping Zhuang, Qian Liang, Xiao-ping Wang, Tong-sheng Chen

Proceedings Volume 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X; 82251Q (2012) https://doi.org/10.1117/12.905786
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

The degenerative joint disease such as osteoarthritis (OA) is closely associated with the death of chondrocytes in apoptosis fashion. Hydrogen peroxide (H₂O₂), higher expression following acute damage in OA patients, has been shown to be up-regulated during apoptosis in a bulk of experimental models. This study was aimed to explore the mechanism of H2O2-induced rabbit chondrocytes apoptosis. Articular cartilage was biopsied from the joints of 6 weeks old New Zealand rabbits. Cell Counting Kit (CCK-8) assay was used to assess the inhibitory effect of H₂O₂ on cell viability. H₂O₂ treatment induced a remarkable reduction of cell viability. We used flow cytometry to assess the form of cell death with Annexin-V/PI double staining, and found that H2O2 treatment induced apoptosis in a dose-and time-dependent manner. Exposure of chondrocytes to 1.5 mM of H₂O₂ for 2 h induced a burst apoptosis that can be alleviated by N-acetyl cysteine (NAC) pretreatment, an anti-oxidant amino-acid derivative. Loss of mitochondria membrane potential (▵Ψm) was evaluated using confocal microscopy imaging and flow cytometry (FCM). H₂O₂ treatment induced a marked reduction of ▵Ψm, and the abrupt disappearance of ▵Ψm occurred within 5 minutes. These results indicate that H₂O₂ induces a rapid apoptosis via a mitochondrial pathway in rabbit chondrocytes.

Citation Download Citation

Cai-ping Zhuang, Qian Liang, Xiao-ping Wang, and Tong-sheng Chen "Hydrogen peroxide induces apoptosis via a mitochondrial pathway in chondrocytes", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82251Q (13 February 2012); https://doi.org/10.1117/12.905786

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