Kynetic resazurin assay (KRA) for bacterial quantification of foodborne pathogens

Yaxal Arenas; Arkady Mandel; Lothar Lilge

doi:10.1117/12.912177

13 February 2012 Kynetic resazurin assay (KRA) for bacterial quantification of foodborne pathogens

Yaxal Arenas, Arkady Mandel, Lothar Lilge

Proceedings Volume 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X; 82252K (2012) https://doi.org/10.1117/12.912177
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Fast detection of bacterial concentrations is important for the food industry and for healthcare. Early detection of infections and appropriate treatment is essential since, the delay of treatments for bacterial infections tends to be associated with higher mortality rates. In the food industry and in healthcare, standard procedures require the count of colony-forming units in order to quantify bacterial concentrations, however, this method is time consuming and reports require three days to be completed. An alternative is metabolic-colorimetric assays which provide time efficient in vitro bacterial concentrations. A colorimetric assay based on Resazurin was developed as a time kinetic assay (KRA) suitable for bacterial concentration measurements. An optimization was performed by finding excitation and emission wavelengths for fluorescent acquisition. A comparison of two non-related bacteria, foodborne pathogens Escherichia coli and Listeria monocytogenes, was performed in 96 well plates. A metabolic and clonogenic dependence was established for fluorescent kinetic signals.

Citation Download Citation

Yaxal Arenas, Arkady Mandel, and Lothar Lilge "Kynetic resazurin assay (KRA) for bacterial quantification of foodborne pathogens", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82252K (13 February 2012); https://doi.org/10.1117/12.912177

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