Spectral variations in narrow band imaging depth-selectivity: mucosal scattering vs. hemoglobin absorption

Quanzeng Wang; Du Le; Jessica C. Ramella-Roman; Joshua Pfefer

doi:10.1117/12.2008768

13 March 2013 Spectral variations in narrow band imaging depth-selectivity: mucosal scattering vs. hemoglobin absorption

Quanzeng Wang, Du Le, Jessica C. Ramella-Roman, Joshua Pfefer

Author Affiliations +

Proceedings Volume 8573, Design and Quality for Biomedical Technologies VI; 85730N (2013) https://doi.org/10.1117/12.2008768
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Spectral variations in contrast enhancement of mucosal vasculature are a key feature of narrow band imaging (NBI) devices. In prior NBI studies, the enhanced visualization of larger, deeper vessels with green light (e.g., 540 nm) relative to violet light (e.g., 415 nm) has often been attributed to the well-known monotonic decrease in scattering coefficient with wavelength in biological tissues. We have developed and implemented numerical and experimental approaches to elucidate and quantify this and other light-tissue interaction effects relevant to NBI. A Monte Carlo model incorporating vessel-like inclusions with a range of diameters (20 to 400 microns) and depths (20 to 400 microns) was used to predict reflectance and fluence distributions in the tissue and calculate vessel contrast values. These results were compared to experimental measurements based on a liquid phantom with a hemoglobin-filled capillary. By comparing results for cases representing mucosa regions with and without blood, we were able to evaluate the relative significance of absorption and scattering on spectral variations in depth-selectivity. Results indicate that at 415 nm, detection of superficial vasculature with NBI was almost entirely dependent on the absorption coefficient of the blood in the vessel of interest. The enhanced visualization of deep vessels at 540 nm bands relative to 415 nm was due primarily to absorption by the superficial vasculature rather than a decrease in scattering coefficient. While computationally intensive, our numerical modeling approach provides unique insights into the light propagation mechanisms underlying this emerging clinical imaging technology.

Citation Download Citation

Quanzeng Wang, Du Le, Jessica C. Ramella-Roman, and Joshua Pfefer "Spectral variations in narrow band imaging depth-selectivity: mucosal scattering vs. hemoglobin absorption", Proc. SPIE 8573, Design and Quality for Biomedical Technologies VI, 85730N (13 March 2013); https://doi.org/10.1117/12.2008768

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Absorption

Scattering

Tissues

Reflectivity

Capillaries

Visualization

Blood

Show All Keywords

Keywords/Phrases

Search In:

Publication Years