Optimized methodology for low-contrast fluorescence recovery using a new approach for reference tracer normalization

Robert W. Holt; Kenneth M. Tichauer; Fadi El-Ghussein; Jason R. Gunn; Frederic Leblond; Brian W. Pogue

doi:10.1117/12.2004829

25 March 2013 Optimized methodology for low-contrast fluorescence recovery using a new approach for reference tracer normalization

Robert W. Holt, Kenneth M. Tichauer, Fadi El-Ghussein, Jason R. Gunn, Frederic Leblond, Brian W. Pogue

Author Affiliations +

Proceedings Volume 8578, Optical Tomography and Spectroscopy of Tissue X; 85781W (2013) https://doi.org/10.1117/12.2004829
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

A main problem with tomographic fluorescence recovery is that it can only reliably recover images of high contrast to background ratio, which is a problematic issue when the fluorescent contrast in a region of interest is near a significant source of background contrast, such as organs of filtration. A method is presented here, combining the resolution of structural image guidance with the benefits of using multiple fluorescent tracers, one targeted to the tumor of interest and one untargeted, in order to substantially improve the accuracy of recovered contrast values for targeted tracer concentration. Using the normalized subtraction in the data space, the recovery of lower contrast regions can be dramatically improved by suppressing the effect of larger perturbations which appear in both the targeted and untargeted fluorescence data sets. This methodology has significant potential value when imaging near excretory organs such as liver, lung, kidneys and bladder, depending upon the agent to be imaged.

Citation Download Citation

Robert W. Holt, Kenneth M. Tichauer, Fadi El-Ghussein, Jason R. Gunn, Frederic Leblond, and Brian W. Pogue "Optimized methodology for low-contrast fluorescence recovery using a new approach for reference tracer normalization", Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 85781W (25 March 2013); https://doi.org/10.1117/12.2004829

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Sensors

Fluorescence tomography

Signal detection

Tumors

Image restoration

Tomography

Show All Keywords

Keywords/Phrases

Search In:

Publication Years