Electric field modulation of the motility of actin filaments on myosin-functionalised surfaces

L. C. Ramsey; J. Aveyard; H. van Zalinge; M. Persson; A. Månsson; D. V. Nicolau

doi:10.1117/12.2010559

19 February 2013 Electric field modulation of the motility of actin filaments on myosin-functionalised surfaces

L. C. Ramsey, J. Aveyard, H. van Zalinge, M. Persson, A. Månsson, D. V. Nicolau

Proceedings Volume 8594, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X; 85940R (2013) https://doi.org/10.1117/12.2010559
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We investigated the difference in electrically guided acto-myosin motility on two surfaces. Rabbit skeletal muscle heavy meromyosin (HMM) was absorbed onto surfaces coated with Nitrocellulose (NC) and Poly(butyl methacrylate) (PBMA). A modified in vitro motility assay with sealed chambers for the insertion of electrodes allowed an electrical field to be applied across the flow cell. On all surfaces a small increase in velocity and general guidance of the actin filaments towards the positive electrode is seen at field strengths in the range of ~3000 – 4000Vm^-1. A large increase in velocity was observed at ~5000Vm^-1 and a significant change in the velocity of the actin filaments present in field strengths higher than this. NC supported the highest percentage of motile filaments and at a field of 8000Vm^-1 reached ~66%. PBMA however supported the least percentage of motile filaments and irregular motility was observed even at higher fields where guidance was expected to be strong. The change in velocity in the range of fields tested varied significantly on the surfaces with NC displaying a 46% increase from 0 to 8000Vm^-1 whereas on PBMA this value was just 37%.

Citation Download Citation

L. C. Ramsey, J. Aveyard, H. van Zalinge, M. Persson, A. Månsson, and D. V. Nicolau "Electric field modulation of the motility of actin filaments on myosin-functionalised surfaces", Proc. SPIE 8594, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X, 85940R (19 February 2013); https://doi.org/10.1117/12.2010559

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In vitro testing

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