Microcavity single virus detection and sizing with molecular sensitivity

V. R. Dantham; S. Holler; V. Kolchenko; Z. Wan; S. Arnold

doi:10.1117/12.2003098

22 February 2013 Microcavity single virus detection and sizing with molecular sensitivity

V. R. Dantham, S. Holler, V. Kolchenko, Z. Wan, S. Arnold

Proceedings Volume 8600, Laser Resonators, Microresonators, and Beam Control XV; 86001P (2013) https://doi.org/10.1117/12.2003098
Event: SPIE LASE, 2013, San Francisco, California, United States

Abstract

We report the label-free detection and sizing of the smallest individual RNA virus, MS2 by a spherical microcavity. Mass of this virus is ~6 ag and produces a theoretical resonance shift ~0.25 fm upon adsorbing an individual virus at the equator of the bare microcavity, which is well below the r.m.s background noise of 2 fm. However, detection was accomplished with ease (S/N = 8, Q = 4x10⁵) using a single dipole stimulated plasmonic-nanoshell as a microcavity wavelength shift enhancer. Analytical expressions based on the “reactive sensing principle” are developed to extract the radius of the virus from the measured signals. Estimated limit of detection for these experiments was ~0.4 ag or 240 kDa below the size of all known viruses, largest globular and elongated proteins [Phosphofructokinase (345 kDa) and Fibrinogen (390 kDa), respectively].

Citation Download Citation

V. R. Dantham, S. Holler, V. Kolchenko, Z. Wan, and S. Arnold "Microcavity single virus detection and sizing with molecular sensitivity", Proc. SPIE 8600, Laser Resonators, Microresonators, and Beam Control XV, 86001P (22 February 2013); https://doi.org/10.1117/12.2003098

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