IFSA: a microfluidic chip-platform for frit-based immunoassay protocols

Nadine Hlawatsch; Michael Bangert; Peter Miethe; Holger Becker; Claudia Gärtner

doi:10.1117/12.2004127

13 March 2013 IFSA: a microfluidic chip-platform for frit-based immunoassay protocols

Nadine Hlawatsch, Michael Bangert, Peter Miethe, Holger Becker, Claudia Gärtner

Proceedings Volume 8615, Microfluidics, BioMEMS, and Medical Microsystems XI; 86150J (2013) https://doi.org/10.1117/12.2004127
Event: SPIE MOEMS-MEMS, 2013, San Francisco, California, United States

Abstract

Point-of-care diagnostics (POC) is one of the key application fields for lab-on-a-chip devices. While in recent years much of the work has concentrated on integrating complex molecular diagnostic assays onto a microfluidic device, there is a need to also put comparatively simple immunoassay-type protocols on a microfluidic platform. In this paper, we present the development of a microfluidic cartridge using an immunofiltration approach. In this method, the sandwich immunoassay takes place in a porous frit on which the antibodies have immobilized. The device is designed to be able to handle three samples in parallel and up to four analytical targets per sample. In order to meet the critical cost targets for the diagnostic market, the microfluidic chip has been designed and manufactured using high-volume manufacturing technologies in mind. Validation experiments show comparable sensitivities in comparison with conventional immunofiltration kits.

Citation Download Citation

Nadine Hlawatsch, Michael Bangert, Peter Miethe, Holger Becker, and Claudia Gärtner "IFSA: a microfluidic chip-platform for frit-based immunoassay protocols", Proc. SPIE 8615, Microfluidics, BioMEMS, and Medical Microsystems XI, 86150J (13 March 2013); https://doi.org/10.1117/12.2004127

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available