Genetic diversity of phytophthoRA sojae isolates in Heilongjiang province in China assessed by RAPD and EST-SSR

J. J. Wu; P. F. Xu; L. J. Liu; J. S. Wang; W. G. Lin; S. Z. Zhang; L. Wei

doi:10.1117/12.2020182

19 March 2013 Genetic diversity of Phytophthora sojae isolates in Heilongjiang Province in China assessed by RAPD and EST-SSR

J. J. Wu, P. F. Xu, L. J. Liu, J. S. Wang, W. G. Lin, S. Z. Zhang, L. Wei

Author Affiliations +

Proceedings Volume 8762, PIAGENG 2013: Intelligent Information, Control, and Communication Technology for Agricultural Engineering; 87620T (2013) https://doi.org/10.1117/12.2020182
Event: Third International Conference on Photonics and Image in Agriculture Engineering (PIAGENG 2013), 2013, Sanya, China

Abstract

Random-amplified polymorphic DNA (RAPD) and EST-SSR markers were used to estimate the genetic relationship among thirty-nine P.sojae isolates from three locations in Heilongjiang Province, and nine isolates from Ohio in America were made as reference strains. 10 of 50 RAPD primers and 5 of 33 EST-SSR were polymorphic across 48 P.sojae isolates. Similarity values among P.sojae isolates were from 49% to 82% based on the RAPD data. The similarities based on EST-SSR markers ranged from 47% to 85%. The genetic diversity revealed by EST-SSR marker analysis was higher than that obtained from RAPD. The similarity matrices for the SSR data and the RAPD data were moderately correlated (r = 0.47). Genetic similarity coefficients were also relatively lower, which demonstrated complicated genetic background within each location. The high similarity values range revealed the ability of RAPD/EST-SSR markers to distinguish even among morphological similar phytophthora.

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J. J. Wu, P. F. Xu, L. J. Liu, J. S. Wang, W. G. Lin, S. Z. Zhang, and L. Wei "Genetic diversity of Phytophthora sojae isolates in Heilongjiang Province in China assessed by RAPD and EST-SSR", Proc. SPIE 8762, PIAGENG 2013: Intelligent Information, Control, and Communication Technology for Agricultural Engineering, 87620T (19 March 2013); https://doi.org/10.1117/12.2020182

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