Direct measurement of formation of loops in DNA by a human tumor suppressor protein

Amy Migliori; Samuel Kung; Danielle Wang; Douglas E. Smith

doi:10.1117/12.2027153

12 September 2013 Direct measurement of formation of loops in DNA by a human tumor suppressor protein

Amy Migliori, Samuel Kung, Danielle Wang, Douglas E. Smith

Proceedings Volume 8810, Optical Trapping and Optical Micromanipulation X; 881030 (2013) https://doi.org/10.1117/12.2027153
Event: SPIE NanoScience + Engineering, 2013, San Diego, California, United States

Abstract

In previous work we developed methods using optical tweezers to measure protein-mediated formation of loops in DNA structures that can play an important role in regulating gene expression. We previously applied this method to study two-site restriction endonucleases, which were convenient model systems for studying this phenomenon. Here we report preliminary work in which we have applied this method to study p53, a human tumor suppressor protein, and show that we can measure formation of loops. Previous biophysical evidence for loops comes from relatively limited qualitative studies of fixed complexes by electron microscopy4. Our results provide independent corroboration and future opportunities for more quantitative studies investigating structure and mechanics.

Citation Download Citation

Amy Migliori, Samuel Kung, Danielle Wang, and Douglas E. Smith "Direct measurement of formation of loops in DNA by a human tumor suppressor protein", Proc. SPIE 8810, Optical Trapping and Optical Micromanipulation X, 881030 (12 September 2013); https://doi.org/10.1117/12.2027153

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