Autofluorescence lifetime metrology for label-free detection of cartilage matrix degradation

Mohammad B. Nickdel; João L. Lagarto; Douglas J. Kelly; Hugh B. Manning; Kazuhiro Yamamoto; Clifford B. Talbot; Christopher Dunsby; Paul French; Yoshifumi Itoh

doi:10.1117/12.2037285

17 March 2014 Autofluorescence lifetime metrology for label-free detection of cartilage matrix degradation

Mohammad B. Nickdel, João L. Lagarto, Douglas J. Kelly, Hugh B. Manning, Kazuhiro Yamamoto, Clifford B. Talbot, Christopher Dunsby, Paul French, Yoshifumi Itoh

Author Affiliations +

Proceedings Volume 8940, Optical Biopsy XII; 89400H (2014) https://doi.org/10.1117/12.2037285
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Degradation of articular cartilage extracellular matrix (ECM) by proteolytic enzyme is the hallmark of arthritis that leads to joint destruction. Detection of early biochemical changes in cartilage before irreversible structural damages become apparent is highly desirable. Here we report that the autofluorescence decay profile of cartilage is significantly affected by proteolytic degradation of cartilage ECM and can be characterised by measurements of the autofluorescence lifetime (AFL). A multidimensional fluorometer utilizing ultraviolet excitation at 355 nm or 375 nm coupled to a fibreoptic probe was developed for single point time-resolved AFL measurements of porcine articular cartilage explants treated with different proteinases. Degradation of cartilage matrix components by treating with bacterial collagenase, matrix metalloproteinase 1, or trypsin resulted in significant reduction of AFL of the cartilage in both a dose and time dependent manner. Differences in cartilage AFL were also confirmed by fluorescence lifetime imaging microscopy (FLIM). Our data suggest that AFL of cartilage tissue is a potential non-invasive readout to monitor cartilage matrix integrity that may be utilized for diagnosis of arthritis as well as monitoring the efficacy of anti-arthritic therapeutic agents.

Citation Download Citation

Mohammad B. Nickdel, João L. Lagarto, Douglas J. Kelly, Hugh B. Manning, Kazuhiro Yamamoto, Clifford B. Talbot, Christopher Dunsby, Paul French, and Yoshifumi Itoh "Autofluorescence lifetime metrology for label-free detection of cartilage matrix degradation", Proc. SPIE 8940, Optical Biopsy XII, 89400H (17 March 2014); https://doi.org/10.1117/12.2037285

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Cartilage

Collagen

Tissues

Fluorescence lifetime imaging

Luminescence

Fluorometers

Scanning electron microscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years