Optical characterization of ex-vivo axillary lymph nodes of breast-cancer patients using a custom-built spectrophotometer

Ashwin Sampathkumar; Emi Saegusa-Beecroft; Jonathan Mamou; Parag V. Chitnis; Junji Machi; Ernest J. Feleppa

doi:10.1117/12.2037864

17 March 2014 Optical characterization of ex-vivo axillary lymph nodes of breast-cancer patients using a custom-built spectrophotometer

Ashwin Sampathkumar, Emi Saegusa-Beecroft, Jonathan Mamou, Parag V. Chitnis, Junji Machi, Ernest J. Feleppa

Author Affiliations +

Proceedings Volume 8940, Optical Biopsy XII; 89400Q (2014) https://doi.org/10.1117/12.2037864
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Quantitative photoacoustics is emerging as a new hybrid modality to investigate diseases and cells in human pathology and cytology studies. Optical absorption of light is the predominant mechanism behind the photoacoustic effect. Therefore, a need exits to characterize the optical properties of specimens and to identify the relevant operating wavelengths for photoacoustic imaging. We have developed a custom low-cost spectrophotometer to measure the optical properties of human axillary lymph nodes dissected for breast-cancer staging. Optical extinction curves of positive and negative nodes were determined in the spectral range of 400 to 1000 nm. We have developed a model to estimate tissue optical properties, taking into account the role of fat and saline. Our results enabled us to select the optimal optical wavelengths for maximizing the imaging contrast between metastatic and noncancerous tissue in axillary lymph nodes.

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Ashwin Sampathkumar, Emi Saegusa-Beecroft, Jonathan Mamou, Parag V. Chitnis, Junji Machi, and Ernest J. Feleppa "Optical characterization of ex-vivo axillary lymph nodes of breast-cancer patients using a custom-built spectrophotometer", Proc. SPIE 8940, Optical Biopsy XII, 89400Q (17 March 2014); https://doi.org/10.1117/12.2037864

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