Fast fluorescence holographic microscopy

Wan Qin; Xiaoqi Yang; Yingying Li; Xiang Peng; Xinghua Qu; Hai Yao; Bruce Z. Gao

doi:10.1117/12.2041839

12 March 2014 Fast fluorescence holographic microscopy

Wan Qin, Xiaoqi Yang, Yingying Li, Xiang Peng, Xinghua Qu, Hai Yao, Bruce Z. Gao

Proceedings Volume 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI; 89491W (2014) https://doi.org/10.1117/12.2041839
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

FINCHSCOPE is a new technology of fluorescence holographic microscopy. It has been successfully applied to recording high-resolution three-dimensional fluorescence images of biological specimens without the need for scanning. In this study, we revealed and analyzed an intrinsic phenomenon, called ghost lens effect, on spatial light modulator which is the core element enabling the incoherent correlation in the FINCHSCOPE. The ghost lens effect can degrade the imaging quality by introducing multiple spherical waves with different focal lengths into the correlation and thus increasing the noise in the recorded holograms.

Citation Download Citation

Wan Qin, Xiaoqi Yang, Yingying Li, Xiang Peng, Xinghua Qu, Hai Yao, and Bruce Z. Gao "Fast fluorescence holographic microscopy", Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 89491W (12 March 2014); https://doi.org/10.1117/12.2041839

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