Proof Of Forster Energy Transfer Between Fad And Fmn In Cytochrome P450 Reductase By Time-Resolved Red-Edge Spectroscopy.

P I.H Bastiaens; P J.M Bonants; A van Hoek; F Muller; A J.W.G Visser

doi:10.1117/12.945398

24 June 1988 Proof Of Forster Energy Transfer Between Fad And Fmn In Cytochrome P450 Reductase By Time-Resolved Red-Edge Spectroscopy.

P I.H Bastiaens, P J.M Bonants, A van Hoek, F Muller, A J.W.G Visser

Author Affiliations +

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945398
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

By use of steady state and time-resolved fluorescence techniques Forster energy transfer between the cofactors, FAD and FMN, in cytochrome-P450 reductase is observed. Red-edge spectroscopy allowed us to assign the actual rate constant of transfer as apparent in the anisotropy decay of the holoenzyme. The same approach was used in biflavinyl compounds entrapped in polymethylmetacrylate. As anticipated the fluorescence depolarisation of this system showed a great similarity with the enzyme system. From the results the interflavin distance in the enzyme was estimated between 1.6 and 2.4 nm.

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P I.H Bastiaens, P J.M Bonants, A van Hoek, F Muller, and A J.W.G Visser "Proof Of Forster Energy Transfer Between Fad And Fmn In Cytochrome P450 Reductase By Time-Resolved Red-Edge Spectroscopy.", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945398

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