Therapeutic and diagnostic set for irradiation the cell lines in low level laser therapy

Lukasz Gryko; Andrzej Zajac; Marian Gilewski; Justyna Szymanska; Krzysztof Goralczyk

doi:10.1117/12.2064293

12 May 2014 Therapeutic and diagnostic set for irradiation the cell lines in low level laser therapy

Lukasz Gryko, Andrzej Zajac, Marian Gilewski, Justyna Szymanska, Krzysztof Goralczyk

Proceedings Volume 9228, Optical Fibers and Their Applications 2014; 92280I (2014) https://doi.org/10.1117/12.2064293
Event: 15th Conference on Optical Fibers and Their Applications, 2014, Lipowy Most, Poland

Abstract

In the paper is presented optoelectronic diagnostic set for standardization the biostimulation procedures performed on cell lines. The basic functional components of the therapeutic set are two digitally controlled illuminators. They are composed of the sets of semiconductor emitters – medium power laser diodes and high power LEDs emitting radiation in wide spectral range from 600 nm to 1000 nm. Emitters are coupled with applicator by fibre optic and optical systems that provides uniform irradiation of vessel with cell culture samples. Integrated spectrometer and optical power meter allow to control the energy and spectral parameters of electromagnetic radiation during the Low Level Light Therapy procedure. Dedicated power supplies and digital controlling system allow independent power of each emitter . It was developed active temperature stabilization system to thermal adjust spectral line of emitted radiation to more efficient association with absorption spectra of biological acceptors. Using the set to controlled irradiation and allowing to measure absorption spectrum of biological medium it is possible to carry out objective assessment the impact of the exposure parameters on the state cells subjected to Low Level Light Therapy. That procedure allows comparing the biological response of cell lines after irradiation with radiation of variable spectral and energetic parameters. Researches were carried out on vascular endothelial cell lines. Cells proliferations after irradiation of LEDs: 645 nm, 680 nm, 740 nm, 780 nm, 830 nm, 870 nm, 890 nm, 970 nm and lasers 650 nm and 830 nm were examined.

Citation Download Citation

Lukasz Gryko, Andrzej Zajac, Marian Gilewski, Justyna Szymanska, and Krzysztof Goralczyk "Therapeutic and diagnostic set for irradiation the cell lines in low level laser therapy", Proc. SPIE 9228, Optical Fibers and Their Applications 2014, 92280I (12 May 2014); https://doi.org/10.1117/12.2064293

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