Lensless microscope based on iterative in-line holographic reconstruction

Jigang Wu

doi:10.1117/12.2071890

11 November 2014 Lensless microscope based on iterative in-line holographic reconstruction

Jigang Wu

Proceedings Volume 9271, Holography, Diffractive Optics, and Applications VI; 927114 (2014) https://doi.org/10.1117/12.2071890
Event: SPIE/COS Photonics Asia, 2014, Beijing, China

Abstract

We propose a lensless microscopic imaging technique based on iteration algorithm with known constraint for image reconstruction in digital in-line holography. In our method, we introduce a constraint on the sample plane as known part in the lensless microscopy for iteration algorithm in order to eliminate the twin-image effect of holography and thus lead to better performance on microscopic imaging. We evaluate our method by numerical simulation and built a prototype in-line holographic imaging system and demonstrated its capability by preliminary experiments. In our proposed setup, a carefully designed photomask used to hold the sample is under illumination of a coherent light source. The in-line hologram is then recorded by a CMOS sensor. In the reconstruction, the known information of the illumination beam and the photomask is used as constraints in the iteration process. The improvement of image quality because of suppression of twin-images can be clearly seen by comparing the images obtained by direct holographic reconstruction and our iterative method.

Citation Download Citation

Jigang Wu "Lensless microscope based on iterative in-line holographic reconstruction", Proc. SPIE 9271, Holography, Diffractive Optics, and Applications VI, 927114 (11 November 2014); https://doi.org/10.1117/12.2071890

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available