Optimized optical clearing method for imaging  central nervous system

Tingting Yu; Yisong Qi; Hui Gong; Qingming Luo; Dan Zhu

doi:10.1117/12.2078736

10 March 2015 Optimized optical clearing method for imaging central nervous system

Tingting Yu, Yisong Qi, Hui Gong, Qingming Luo, Dan Zhu

Proceedings Volume 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II; 930527 (2015) https://doi.org/10.1117/12.2078736
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The development of various optical clearing methods provides a great potential for imaging entire central nervous system by combining with multiple-labelling and microscopic imaging techniques. These methods had made certain clearing contributions with respective weaknesses, including tissue deformation, fluorescence quenching, execution complexity and antibody penetration limitation that makes immunostaining of tissue blocks difficult. The passive clarity technique (PACT) bypasses those problems and clears the samples with simple implementation, excellent transparency with fine fluorescence retention, but the passive tissue clearing method needs too long time. In this study, we not only accelerate the clearing speed of brain blocks but also preserve GFP fluorescence well by screening an optimal clearing temperature. The selection of proper temperature will make PACT more applicable, which evidently broaden the application range of this method.

Citation Download Citation

Tingting Yu, Yisong Qi, Hui Gong, Qingming Luo, and Dan Zhu "Optimized optical clearing method for imaging central nervous system", Proc. SPIE 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II, 930527 (10 March 2015); https://doi.org/10.1117/12.2078736

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KEYWORDS

Brain

Luminescence

Photoacoustic tomography

Tissues

Transparency

Green fluorescent protein

Imaging systems

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