Absorption and fluorescence spectroscopy on a smartphone

Md. Arafat Hossain; John Canning; Kevin Cook; Sandra Ast; Peter J. Rutledge; Abbas Jamalipour

doi:10.1117/12.2184354

1 July 2015 Absorption and fluorescence spectroscopy on a smartphone

Md. Arafat Hossain, John Canning, Kevin Cook, Sandra Ast, Peter J. Rutledge, Abbas Jamalipour

Proceedings Volume 9655, Fifth Asia-Pacific Optical Sensors Conference; 96551Z (2015) https://doi.org/10.1117/12.2184354
Event: Fifth Asia Pacific Optical Sensors Conference, 2015, Jeju, Korea, Republic of

Abstract

A self-powered smartphone-based field-portable “dual” spectrometer has been developed for both absorption and fluorescence measurements. The smartphone’s existing flash LED has sufficient optical irradiance to undertake absorption measurements within a 3D-printed case containing a low cost nano-imprinted polymer diffraction grating. A UV (λ_ex ~ 370 nm) and VIS (λ_ex ~ 450 nm) LED are wired into the circuit of the flash LED to provide an excitation source for fluorescence measurements. Using a customized app on the smartphone, measurements of absorption and fluorescence spectra are demonstrated using pH-sensitive and Zn²⁺-responsive probes. Detection over a 300 nm span with 0.42 nm/pixel spectral resolution is demonstrated. Despite the low cost and small size of the portable spectrometer, the results compare well with bench top instruments.

Citation Download Citation

Md. Arafat Hossain, John Canning, Kevin Cook, Sandra Ast, Peter J. Rutledge, and Abbas Jamalipour "Absorption and fluorescence spectroscopy on a smartphone", Proc. SPIE 9655, Fifth Asia-Pacific Optical Sensors Conference, 96551Z (1 July 2015); https://doi.org/10.1117/12.2184354

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