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7 March 2022 Sub-surface fluorescence time-of-flight imaging using a large format SPAD sensor
Arthur Pétusseau, Petr Bruza, Arin Ulku, Samuel Streeter, Kimberley Samkoe, Claudio Bruschini, Edoardo Charbon, Brian Pogue
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Proceedings Volume PC11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII; PC1194304 (2022) https://doi.org/10.1117/12.2607719
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Classical fluorescence guided surgery uses long laser pulses to excite fluorescent tracers, only providing the practitioner with 2D information. In this work, we develop and test a 3D imaging technique using LiDAR technology to sense target tissue depths up to 5 mm below the surface. Imaging through highly scattering medium like tissues implies that temporal information used for 3D reconstruction is convolved with diffuse dynamics of the medium. Using diffuse reflectance as an indicator of the medium’s optical properties, correction of the time-of-flight is made possible. Time-of-flight fluorescence imaging, captures intensity as well as temporal profile of fluorescence excited by ultra-short pulses.
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Arthur Pétusseau, Petr Bruza, Arin Ulku, Samuel Streeter, Kimberley Samkoe, Claudio Bruschini, Edoardo Charbon, and Brian Pogue "Sub-surface fluorescence time-of-flight imaging using a large format SPAD sensor", Proc. SPIE PC11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII, PC1194304 (7 March 2022); https://doi.org/10.1117/12.2607719
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KEYWORDS
Luminescence
Scattering
Sensors
Time of flight imaging
Laser scattering
Light scattering
Tissues
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