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2 March 2022 Studying membrane biophysics using Graphene-induced energy-transfer
Ingo Gregor, Arindam Ghosh, Tao Chen, Sufi O. Raja, Alexey I. Chizhik, Christoph F. Schmidt, Jörg Enderlein
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Proceedings Volume PC11967, Single Molecule Spectroscopy and Superresolution Imaging XV; PC1196707 (2022) https://doi.org/10.1117/12.2616863
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Graphene-induced energy transfer (GIET) was recently introduced for sub-nanometric axial localization of fluorescent molecules. GIET exploits the near-field energy transfer from an excited fluorophore to a single sheet of graphene. This alters the fluorescence decay-time of the emitter and can be easily determined by fluorescence lifetime imaging microscopy (FLIM). The axial resolution of GIET implies to study of biological membranes. We present the measurement of the thickness of synthetic model membranes and demonstrate changes upon the addition of cholesterol. Furthermore, we are able to show the flipping of lipids from one leaflet to the other and determine the rates of this dynamics. In addition, we used GIET for mapping quasi-stationary states of the mitochondrial membranes before and during ATP synthesis. Upon activation, the inner membrane clearly approaches the outer membrane and the inter-membrane space is reduced by ∼2 nm.
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Ingo Gregor, Arindam Ghosh, Tao Chen, Sufi O. Raja, Alexey I. Chizhik, Christoph F. Schmidt, and Jörg Enderlein "Studying membrane biophysics using Graphene-induced energy-transfer", Proc. SPIE PC11967, Single Molecule Spectroscopy and Superresolution Imaging XV, PC1196707 (2 March 2022); https://doi.org/10.1117/12.2616863
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KEYWORDS
Biophysics
Energy transfer
Acquisition tracking and pointing
Fluorescence lifetime imaging
Molecules
Microscopy
Molecular energy transfer
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