Label-free determination of embryo viability using artificial confocal microscopy (ACM)

Neha Goswami; Rachel B. Arcanjo; Nastasia Z. E. Lai; Xi Chen; Romana A. Nowak; Gabriel Popescu

doi:10.1117/12.2615272

2 March 2022 Label-free determination of embryo viability using artificial confocal microscopy (ACM)

Neha Goswami, Rachel B. Arcanjo, Nastasia Z. E. Lai, Xi Chen, Romana A. Nowak, Gabriel Popescu

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Proceedings Volume PC11970, Quantitative Phase Imaging VIII; PC119700C (2022) https://doi.org/10.1117/12.2615272
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Three-dimensional (3D) cellular systems have been increasingly adopted over 2D cell monolayers to study disease mechanisms and discover drug therapeutics, as they more accurately recapitulate the in vivo cellular communication and development of extracellular matrices. However, significant challenges exist for the existing optical microscopy techniques when applied to increasingly thick tissue structures. Recently, we have reported artificial confocal microscopy (ACM), a laser scanning QPI system combined with deep learning algorithms, which renders “synthetic” fluorescence confocal images from thick unlabeled specimens. Here, we aim to determine quantitative markers that can report on the viability of mammalian embryos. We start with the identification of nuclei in the embryonic cells, as the presence of anucleated cells indicate low viability of the embryos. Using phase imaging with computational specificity (PICS), we show that nuclear mask can be predicted from GLIM images alone.

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Neha Goswami, Rachel B. Arcanjo, Nastasia Z. E. Lai, Xi Chen, Romana A. Nowak, and Gabriel Popescu "Label-free determination of embryo viability using artificial confocal microscopy (ACM)", Proc. SPIE PC11970, Quantitative Phase Imaging VIII, PC119700C (2 March 2022); https://doi.org/10.1117/12.2615272

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KEYWORDS

Phase imaging

Confocal microscopy

Microscopy

Cancer

Tissue optics

Biomedical optics

Multiphoton microscopy

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