Multiplexed bio-assay for a reliable detection of HER2 in human plasma using a combined label-free/fluorescence biosensing platform

Tommaso Pileri; Alberto Sinibaldi; Matteo Allegretti; Norbert Danz; Peter Munzert; Elena Giordani; Agostino Occhicone; Frank Sonntag; Patrizio Giacomini; Francesco Michelotti

doi:10.1117/12.2623466

4 March 2022 Multiplexed bio-assay for a reliable detection of HER2 in human plasma using a combined label-free/fluorescence biosensing platform

Tommaso Pileri, Alberto Sinibaldi, Matteo Allegretti, Norbert Danz, Peter Munzert, Elena Giordani, Agostino Occhicone, Frank Sonntag, Patrizio Giacomini, Francesco Michelotti

Author Affiliations +

Proceedings Volume PC11979, Frontiers in Biological Detection: From Nanosensors to Systems XIV; PC1197903 (2022) https://doi.org/10.1117/12.2623466
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Human epidermal growth factor receptor 2 (HER2) over-expression occurs in 15–20% of breast cancers and it is generally associated with a dismal prognosis. In this work, we report on the use of one-dimensional photonic crystal biochips to detect clinically relevant concentrations of HER2 in human plasma samples. To this aim, we optimized an optical read-out system, combining both label-free and fluorescence detection, which makes use of biochips tailored with specific proteins for specific biological recognition. Our biochips were used to discriminate HER2 positive/negative human plasma samples providing a solid and reliable tool for clinical diagnostics.

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Tommaso Pileri, Alberto Sinibaldi, Matteo Allegretti, Norbert Danz, Peter Munzert, Elena Giordani, Agostino Occhicone, Frank Sonntag, Patrizio Giacomini, and Francesco Michelotti "Multiplexed bio-assay for a reliable detection of HER2 in human plasma using a combined label-free/fluorescence biosensing platform", Proc. SPIE PC11979, Frontiers in Biological Detection: From Nanosensors to Systems XIV, PC1197903 (4 March 2022); https://doi.org/10.1117/12.2623466

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KEYWORDS

Plasma

Biosensing

Multiplexing

Breast cancer

Cancer

Oncology

Proteins

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