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4 March 2022 Photostability of red fluorescent proteins under multiphoton excitation
Mikhail Drobizhev, Rosana S. Molina, Jacob Franklin
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Proceedings Volume PC11979, Frontiers in Biological Detection: From Nanosensors to Systems XIV; PC119790G (2022) https://doi.org/10.1117/12.2610082
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Red fluorescent proteins (RFPs) and biosensors built upon them provide an attractive advantage for two-photon laser microscopy because they can report from deeper layers of tissue compared to green fluorescent proteins. Using mCherry RFP we show that although the shorter wavelength excitation (740–800 nm) is several times more efficient compared to longer wavelength excitation (1000–1200 nm), the photobleaching of chromophore occurs much faster for the former. This can be explained by a different photobleaching mechanism: with higher energy-photons, 3-4 photons are sufficient to reach an energy threshold (ionization potential) and photodetach an electron from the chromophore.
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Mikhail Drobizhev, Rosana S. Molina, and Jacob Franklin "Photostability of red fluorescent proteins under multiphoton excitation", Proc. SPIE PC11979, Frontiers in Biological Detection: From Nanosensors to Systems XIV, PC119790G (4 March 2022); https://doi.org/10.1117/12.2610082
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KEYWORDS
Fluorescent proteins
Chromophores
Tin
Microscopes
Microscopy
Multiphoton microscopy
Photons
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