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3 January 2023 Transport of intensity diffraction tomography for three-dimensional label-free microscopy under arbitrary non-matched illumination condition
Jiaji Li, Chao Zuo, Qian Chen
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Proceedings Volume PC12316, Advanced Optical Imaging Technologies V; PC1231607 (2023) https://doi.org/10.1117/12.2643039
Event: SPIE/COS Photonics Asia, 2022, Online Only
Abstract
We present a new label-free three-dimensional (3D) microscopy technique, termed transport of intensity diffraction tomography with non-interferometric synthetic aperture (TIDT-NSA). Without resorting to interferometric detection, TIDT-NSA retrieves the 3D refractive index (RI) distribution of biological specimens from 3D intensity-only measurements at various illumination angles, allowing incoherent-diffraction-limited quantitative 3D phase-contrast imaging. The unique combination of z-scanning the sample with illumination angle diversity in TIDT-NSA provides strong defocus phase contrast and better optical sectioning capabilities suitable for high-resolution tomography of complex multi-layer biological samples. Based on an off-the-shelf bright-field microscope with a programmable lightemitting-diode (LED) illumination source, we demonstrate the achievable imaging resolution of TIDT-NSA at 206 nm laterally and 0.52 um axially with a high-NA oil immersion objective and validate the 3D R
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Jiaji Li, Chao Zuo, and Qian Chen "Transport of intensity diffraction tomography for three-dimensional label-free microscopy under arbitrary non-matched illumination condition", Proc. SPIE PC12316, Advanced Optical Imaging Technologies V, PC1231607 (3 January 2023); https://doi.org/10.1117/12.2643039
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KEYWORDS
Tomography
Diffraction
Microscopy
3D metrology
3D image processing
Biomedical optics
Optical tomography
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