Low- and high-resolution volumetric dynamic optical coherence tomography of in vitro cell cultures

Rion Morishita; Ibrahim Abd El-Sadek; Pradipta Mukherjee; Tomoko Mori; Suzuki Toshio; Antonia Lichtengger; Lim Yi Heng; Zhu Yiqiang; Shuichi Makita; Yuki Yamamoto; Tetsuharu Nagamoto; Satoshi Matsusaka; Yoshiaki Yasuno

doi:10.1117/12.2652167

9 March 2023 Low- and high-resolution volumetric dynamic optical coherence tomography of in vitro cell cultures

Rion Morishita, Ibrahim Abd El-Sadek, Pradipta Mukherjee, Tomoko Mori, Suzuki Toshio, Antonia Lichtengger, Lim Yi Heng, Zhu Yiqiang, Shuichi Makita, Yuki Yamamoto, Tetsuharu Nagamoto, Satoshi Matsusaka, Yoshiaki Yasuno

Author Affiliations +

Proceedings Volume PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII; PC123671D (2023) https://doi.org/10.1117/12.2652167
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We demonstrate high-resolution (3.8-µm axial and 4.8-µm lateral) three-dimensional dynamic (D-) OCT imaging by 840-nm spectral domain OCT, and compare it with a low-resolution 1.3-µm D-OCT. The D-OCT images are obtained by logarithmic-intensity-variance method, which is sensitive to the magnitude of signal fluctuation. Human-induced-pluripotent-stem-cell derived alveolar (lung) organoids and human breast cancer (MCF-7) spheroids were examined. The high-resolution D-OCT revealed the tessellation of high and low dynamics at the matured alveolar epithelium. It is also found that such matured alveolar epithelium exhibits ragged inner surface. For the spheroids, high-scattering spots with low dynamics were observed only in the high-resolution image.

Conference Presentation

Citation Download Citation

Rion Morishita, Ibrahim Abd El-Sadek, Pradipta Mukherjee, Tomoko Mori, Suzuki Toshio, Antonia Lichtengger, Lim Yi Heng, Zhu Yiqiang, Shuichi Makita, Yuki Yamamoto, Tetsuharu Nagamoto, Satoshi Matsusaka, and Yoshiaki Yasuno "Low- and high-resolution volumetric dynamic optical coherence tomography of in vitro cell cultures", Proc. SPIE PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII, PC123671D (9 March 2023); https://doi.org/10.1117/12.2652167

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Optical coherence tomography

In vitro testing

3D modeling

Cancer

Drug development

Lung

Medical research

Show All Keywords

Keywords/Phrases

Search In:

Publication Years