Label-free dynamic OCT (D-OCT) based evaluation of tumor spheroids

Ibrahim G. Abd El-Sadek; Rion Morishita; Tomoko Mori; Shuichi Makita; Pradipta Mukherjee; Satoshi Matsusaka; Yoshiaki Yasuno

doi:10.1117/12.2651543

27 March 2023 Label-free dynamic OCT (D-OCT) based evaluation of tumor spheroids

Ibrahim G. Abd El-Sadek, Rion Morishita, Tomoko Mori, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, Yoshiaki Yasuno

Author Affiliations +

Proceedings Volume PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII; PC123671O (2023) https://doi.org/10.1117/12.2651543
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

Cancers is one of the most fatal diseases threatening the survival rate of humans. The recent advances in the cell culture methods allow cultivation of human-derived tumor cells as a 3-D culture, so-called tumor spheroid, which closely emulates the in vivo solid tumors. Hence, it can be used for anti-cancer drug testing, where the efficacy of anti-cancer drugs is evaluated by the drug-induced morphological and viability alterations of the spheroids. The morphological and tissue viability alterations can be evaluated by using staining histology, bright field microscopy, and fluorescence microscopy. However, these methods have several limitations. First, most of them use chemical labeling and/or tissue slicing, and hence, they are invasive. Second, they are 2-D methods. And hence, volumetric morphology, such as alteration of spheroid volume, cannot be evaluated. Third, their penetration-depth is limited to a few hundred microns. It prevents the imaging of thick tissues, such as spheroid. To overcome the above mentioned limitations, we developed a completely label-free 3-D dynamic optical coherence tomography (D-OCT) method for intracellular motility evaluation of tumor spheroid. To validate the utility of our D-OCT method, we organized several studies, including time-course and drug-response evaluations of human breast cancer (MCF-7 cell-line) spheroids. The 3-D morphological and cell viability alterations of MCF-7 spheroid during time-course and under the application of several anti-cancer drugs have been successfully visualized and quantified using D-OCT. The results suggest that D-OCT might be a useful for tumor spheroid-based drug testing and it might be a useful tool for precision medicine research.

Citation Download Citation

Ibrahim G. Abd El-Sadek, Rion Morishita, Tomoko Mori, Shuichi Makita, Pradipta Mukherjee, Satoshi Matsusaka, and Yoshiaki Yasuno "Label-free dynamic OCT (D-OCT) based evaluation of tumor spheroids", Proc. SPIE PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII, PC123671O (27 March 2023); https://doi.org/10.1117/12.2651543

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available