PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
We report an arrayed optofluidic imaging platform that allows multiplexed image-based cell assay at ultrahigh imaging throughput. The assay platform, consisting of 96 fluidic sample chambers arranged in a circular symmetry, operates in a reconfigurable spinning motion synchronized with an ultrafast laser-scanning microscope (a line-scan rate >10 MHz). Based on a stable through-focus spinning mechanism, the assay platform allows ultralarge field-of-view imaging (18.8 cm2, >160 Gpixels) with high image fidelity and subcellular resolution (in both the bright-field and quantitative phase image contrasts). We further validated that the continuous spinning operation has minimal impact on the cell morphology and viability.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Man Lai Wong, Dickson M. D. Siu, Sum Lau, Kenneth K. Y. Wong, Kevin K. M. Tsia, "Spinning arrayed optofluidic imaging for large-scale single-cell image-based analysis (Conference Presentation)," Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC123900G (16 March 2023); https://doi.org/10.1117/12.2655551