Organelle dynamics imaged label-free with interface self-referencing dynamic full-field optical coherence tomography (Conference Presentation)

Tual Monfort; Salvatore Azzollini; Olivier Thouvenin; Kate Grieve

doi:10.1117/12.2648276

16 March 2023 Organelle dynamics imaged label-free with interface self-referencing dynamic full-field optical coherence tomography (Conference Presentation)

Tual Monfort, Salvatore Azzollini, Olivier Thouvenin, Kate Grieve

Author Affiliations +

Proceedings Volume PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; PC1239110 (2023) https://doi.org/10.1117/12.2648276
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We present a new dynamic full-field optical coherence tomography (D-FF-OCT) imaging technique, which is self-referenced, vibration-insensitive and overcomes D-FF-OCT limitations near specular surfaces, named interface self-referencing D-FF-OCT (iSR-D-FF-OCT). In fibroblasts, retinal organoids, and corneal explants, we demonstrate imaging in the vicinity of reflective interfaces without fringe artefacts, in a live, label-free, non-invasive manner with sub-diffraction limited sectioning capacity within 3D samples. iSR-(D)-FF-OCT has a homogeneous contrast across the imaging field enabling flawless mosaicking. iSR-(D)-FF-OCT complements and is easily implemented on existing D-FF-OCT set-ups to cover a zero to several hundred micrometer depth range.

Conference Presentation

Citation Download Citation

Tual Monfort, Salvatore Azzollini, Olivier Thouvenin, and Kate Grieve "Organelle dynamics imaged label-free with interface self-referencing dynamic full-field optical coherence tomography (Conference Presentation)", Proc. SPIE PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, PC1239110 (16 March 2023); https://doi.org/10.1117/12.2648276

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KEYWORDS

Optical coherence tomography

Interfaces

Glasses

Coherence imaging

3D image processing

Cornea

Image resolution

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