Characterizing extracellular network pore size and microstructural dynamics with Laser Speckle Microrheology (LSM) (Conference Presentation)

Nichaluk Leartprapun; Brandon C. Matthews; Seemantini K. Nadkarni

doi:10.1117/12.2650941

16 March 2023 Characterizing extracellular network pore size and microstructural dynamics with Laser Speckle Microrheology (LSM) (Conference Presentation)

Nichaluk Leartprapun, Brandon C. Matthews, Seemantini K. Nadkarni

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Proceedings Volume PC12393, Biomedical Applications of Light Scattering XIII; PC123930A (2023) https://doi.org/10.1117/12.2650941
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Cells in vivo exist in a 3D porous network of proteins that lend structural support while permitting cellular attachment and migration. Characterization of pore size and microstructural network dynamics is imperative to the biophysical study of cell-ECM interactions and for tissue-engineering applications. We implemented Laser Speckle Microrheology at sub-MHz frequencies to measure mean square displacement (MSD) and its log-log derivative (α) of particles embedded in purified fibrin clots. The power law behavior of the time-dependent MSD provides a measure of pore sizes spanning different particle-to-pore size ratios, relevant for advancing our understanding of cell-ECM interactions.

Conference Presentation

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Nichaluk Leartprapun, Brandon C. Matthews, and Seemantini K. Nadkarni "Characterizing extracellular network pore size and microstructural dynamics with Laser Speckle Microrheology (LSM) (Conference Presentation)", Proc. SPIE PC12393, Biomedical Applications of Light Scattering XIII, PC123930A (16 March 2023); https://doi.org/10.1117/12.2650941

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KEYWORDS

Speckle

Particles

3D metrology

Cell death

Confocal microscopy

Diffusion

In vivo imaging

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