Romuald Houdré,1 Nicolas Villa,1 Enrico Tartari,1 Simon Glicenstein,2 Emmanuel Picard,2 Pierre Marcouxhttps://orcid.org/0000-0003-3855-9662,3,4 Marc Zelsmann,3,4 Grégory Resch,5 Emmanuel Hadji3,6,7
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We will show results towards a new method to perform antibiotic or phage susceptibility test with single phages or bacteria trapped in an optical chip. The system used is a photonic chip containing photonic crystal hollow cavities topped by a microfluidic system allowing the transport of bacteria and phages. We will report the optical signature of the trapping of bacteria and phages in the transmitted light exciting the optical trap. This allows the distinction of different phages families as well as the level of stress or death of a single bacteria in the presence of antibiotics or phages.
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Romuald Houdré, Nicolas Villa, Enrico Tartari, Simon Glicenstein, Emmanuel Picard, Pierre Marcoux, Marc Zelsmann, Grégory Resch, Emmanuel Hadji, "On-chip optical nanotweezers for bacteria and bacteriophages viruses trapping and susceptibility testing," Proc. SPIE PC12649, Optical Trapping and Optical Micromanipulation XX, PC1264908 (5 October 2023); https://doi.org/10.1117/12.2676639