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Our optical positioning and linking (OPAL) platform combines optical tweezers and a biochemical linking mechanism to assemble complex 3D microstructures that can incorporate particles of different sizes and materials and augment existing devices. Updates to system components have increased the maximum speed with which particles can be moved while implementation of a 3D calibration procedure has decreased positional errors. Collecting and processing the backscattered signal using a quadrant photodiode enables classification of the trap particle population and is key for a fully automated assembly process. These updates will permit rapid prototyping and fabrication of even larger, more functional structures.
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